DNA testing conducted by the U.S. Fish and Wildlife Service confirmed the animal was a western Great Lakes wolf, a subspecies of the gray wolf.
Ron Kaufman, a spokesman for the Kansas Department of Wildlife, Parks and Tourism, says officials haven't determined if the wolf was wild or had been in captivity.
The Hays Daily News reported Monday the last confirmed killing of a gray wolf in Kansas was during the winter of 1938-39.
The animal's body has been turned over to the federal wildlife agency. Gray wolves are a protected species outside extreme northern United States.
The hunters have not been identified.